Summary Spermatogenesis is driven by dramatic changes in chromatin regulation, gene transcription, and protein expression. To assess the mechanistic bases for these developmental changes, we utilized multiomic single-cell/nucleus RNA sequencing (sc/snRNA-seq) and single-nucleus assay for transposase-accessible chromatin with sequencing (snATAC-seq) … Read more
Author Archives: cfriese
Thomas Jefferson University Receives $1.25M Grant to Tackle Bone Destruction in Metastatic Breast Cancer
Grant from the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation aims to develop innovative therapies for patients with breast cancer bone metastases. Karen Bussard, PhD, associate professor in the Department of Pharmacology, Physiology, and Cancer Biology at Thomas … Read more
Engineered PsCas9 enables therapeutic genome editing in mouse liver with lipid nanoparticles
Clinical implementation of therapeutic genome editing relies on efficient in vivo delivery and the safety of CRISPR-Cas tools. Previously, we identified PsCas9 as a Type II-B family enzyme capable of editing mouse liver genome upon adenoviral delivery without detectable off-targets … Read more
Foundation funds research to block drug resistance in cancer treatment
The Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation has funded an ambitious initiative to overcome one of the most perplexing and frustrating mysteries of cancer treatment — how to prevent drug resistance. Patients treated for cancers can have … Read more
A naturally occurring alternative to antibiotics? San Antonio Medical Foundation-funded research provides key assistance.
Phages, which live in the digestive tract, could treat bacterial infections if issues impacting effectiveness are solved. Research at UT Health San Antonio and UTSA is doing just that. Contact: Will Sansom, 210-567-2579, sansom@uthscsa.edu SAN ANTONIO (Aug. 30, 2023) — … Read more
A Polarity-Sensitive Far-Red Fluorescent Probe for Glucose Sensing through Skin
Lydia Colvin, Dandan Tu, Darin Dunlap, Alberto Rios, and Gerard Coté Abstract The field of glucose biosensors for diabetes management has been of great interest over the past 60 years. Continuous glucose monitoring (CGM) is important to continuously track the glucose level to provide better … Read more
RNA targeting unleashes indiscriminate nuclease activity of CRISPR–Cas12a2
Abstract Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded DNA following recognition of a complementary RNA target, culminating in abortive infection1. Here we report structures of Cas12a2 in binary, ternary and … Read more
Evidence for reduced BRCA2 functional activity in Homo sapiens after divergence from the chimpanzee-human last common ancestor
Link to article https://www.cell.com/cell-reports/pdfExtended/S2211-1247(22)00535-6 Memorial Sloan Kettering Statement BRCA mutations have been implicated in pancreatic cancer, as well as breast and ovarian cancers. Dr. Iacobuzio-Donahue and her colleagues traced the BRCA2 gene back to the chimpanzee-human last common ancestor in … Read more
Reestablishment of spermatogenesis after more than 20 years of cryopreservation of rat spermatogonial stem cells reveals an important impact in differentiation capacity
Abstract Treatment of cancer in children is increasingly successful but leaves many prepubertal boys suffering from infertility or subfertility later in life. A current strategy to preserve fertility in these boys is to cryopreserve a testicular biopsy prior to treatment … Read more
Structural basis for mismatch surveillance by CRISPR–Cas9
Abstract CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage1,2,3,4, and the underlying mechanisms by which Cas9 recognizes mismatches are poorly understood5,6,7. Although Cas9 variants with greater discrimination against mismatches have been designed8,9,10, these suffer from … Read more
