Abstract: We present tetramer-associated T-cell receptor sequencing (TetTCR-seq) to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-seq to identify patterns in TCR cross-reactivity with cancer neoantigens and to rapidly isolate neoantigen-specific TCRs with no cross-reactivity to the wildtype antigen.
Zhang S.Q., Ma K.Y., Schonnesen A., Zhang M., He C., Sun E., Williams C., Jia W., Jiang N., “High-throughput determination of the antigen specificities of T cell receptors in single cells”, Nature Biotechnology, 2018.
Dr. Jiang’s work was supported in part by the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation.